Time-resolved CIDNP study of protein-related molecules
نویسندگان
چکیده
Time-resolved CIDNP and Laser Flash Photolysis have been applied to the investigation of the kinetics and mechanism of CIDNP formation in the reactions of aza-aromatic dye 2,2′-dipyridyl and protein-related molecules, including N-acetyl derivatives of three amino acids (tryptophan, tyrosine, histidine) the dipeptide tryptophan-tyrosine, and hen egg-white lysozyme in native and denatured states. CIDNP kinetics for the dipeptide is affected by intramolecular electron transfer from tyrosine residue to the oxidized tryptophan residue. The peculiarities of CIDNP kinetic behavior in the presence of IET are studied for the denatured state of lysozyme. In the native state, IET was found to be ineffective.
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